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The Carol Moss Spivak Cell Imaging Facility is a service of the UCLA Brain Research Institute for the implementation of biological confocal and 2-photon laser-scanning microscopy and some associated technologies. The Facility has a new Leica TCS SP MP Inverted Confocal and 2-Photon Laser-Scanning Microscope, a new Leica TCS SP MP Fixed-Stage Upright Confocal and 2-Photon Laser-Scanning Microscope and a Carl Zeiss LSM 310 laser-scanning confocal microscope, PC and Macintosh computers for image processing and a dye sublimation printer and a film graphics recorder for confocal data output. Some basic equipment is available for physiological experiments involving confocal and 2-photon scanning. These include an Axon Instruments Axopatch 200B Patch Amplifier, a Burleigh micromanipulator, and a Hammamatsu video camera for IR-DIC imaging.

The Director of the Carol Moss Spivak Cell Imaging Center is Dr. Matt Schibler; 310-983-1077; alms@cnsi.ucla.edu

The following links have been set up to provide some background in microscopy and to help you make the most of your use of the core facilities and the resources in equipment, services and personnel available to assist members of the Brain Research Institute and the UCLA biomedical community in the area of microscopic imaging.  We have included practical information about  instrumentation and  microscopic theory and techniques as a basis for the use of microscopes in the core facilities as well as in your own laboratories. 

The Basics of Light Microscopy

Transmitted Light Microscopy

Fluorescence Microscopy

Confocal Microscopy

    2-Photon Laser Scanning Microscopy

 

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