Site Search

 

Dark field microscopy is a method which also creates contrast between the object and the surrounding field. As the name implies, the background is dark and the object is bright. A annular stop is also used for dark field, but the stop is now outside the field of view. Only light coming from the outside of the beam passes through the object and it cannot be seen directly. Only when light from the stop is deflected and deviated by the object can it be seen. This method also produces a great deal of glare and therefore the specimen often appears as a bright silhouette rather than as a bright object of which much detail can be determined. The following diagram shows the setup of the dark field light path.

(Redrawn from Gray)*
Please remember that when using dark field optics, that any dirt or dust or other garbage on your slide or specimen will shine just as brightly as your object of interest. Make sure your slide is immaculately clean.
*Diagram redrawn from Gray , P. 1964. Handbook of Basic Microtechnique McGraw-Hill: New York.
 

Upcoming EventsSupport the BRI!

Upcoming EventsNEUROSCIENCE NEWS HIGHLIGHTS
Current Edition of Neuroscience News

Upcoming EventsFEATURED ARTICLE
Sleep well, breathe easy - Distinguished Professor Ronald Harper to deliver 21st annual H.W. Magoun Lecture

BRI NewsIN THE NEWS
 One Animal Researcher Refuses to Hide

 BRI News Archive

 Newsroom.ucla.edu

Upcoming EventsUPCOMING EVENTS

 Joint Seminars in Neuroscience

 Neuroscience Seminars biweekly calendar