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Steven G. Clarke, Ph.D.
Distinguished Professor, Chemistry and Biochemistry
Member, Brain Research Institute
Graduate Program in Biochemistry, Molecular and Structural Biology
Awards and Honors:Princeton University Visiting Fellow, Princeton University American Chemical Society American Chemical Society/Ralph F. Hirschmann Award in Peptide Chemistry Alfred P. Sloan Research Fellowship in Chemistry Alfred P. Sloan Research Fellowship in Chemistry Harvard NSF Predoctoral Fellow NIH MERIT Award Pomona College Wilson Prize in Chemistry UCLA Academic Senate Distinguished Teaching Award, including the Eby Award for the Art of Teaching UCLA Academic Senate Distinguished Teaching Award, including the Eby Award for the Art of Teaching Miller Research Fellow, UC Berkeley Miller Research Fellow, UC Berkeley Joseph F. Foster Memorial Lecturer, Purdue University
Lab Number: (310) 825-3137 & 825-8121
Work Phone Number: (310) 825-8754
Work Phone Number: (310) 825-8754
Laboratory Address:Paul Boyer Hall 628, 637 & 638
Work Address:Paul Boyer Hall 640
Steven Clarke has been on the faculty of the UCLA Department of Chemistry and Biochemistry since 1978. He is currently a Distinguished Professor of Biochemistry and Director of the UCLA Cellular and Molecular Biology Training Program. He was born in Los Angeles and attended public schools in Altadena and Pasadena, California. He did his undergraduate work at Pomona College in Claremont, majoring in Chemistry and Zoology. During this time, he did undergraduate research at the UCLA Brain Research Institute with Dr. James E. Skinner and Professor Donald Lindsley on neural mechanisms of attention. He was also an NIH fellow in the laboratory of Dr. Peter Mitchell at Glynn Research Laboratories in Bodmin, England studying mitochondrial amino acid transport. He obtained his PhD in Biochemistry and Molecular Biology at Harvard University working as an NSF Fellow with Professor Guido Guidotti on membrane protein-detergent interactions and the identification of the major rat liver mitochondrial polypeptides as enzymes of the urea cycle. He returned to California to do postdoctoral work as a Miller Fellow at the University of California, Berkeley, with Professor Dan Koshland, identifying membrane receptors for bacterial chemotaxis. His research at UCLA has focused on roles of novel protein methyltransferases in aging and biological regulation highlighted by discoveries of the protein L-isoaspartyl repair methyltransferase, the isoprenylcysteine protein methyltransferase, and the protein phosphatase 2A methyltransferase. He has been a visiting scholar at Princeton University (1986-87), the University of Washington (2004-2005), and Vanderbilt University (2015).
Warmack Rebeccah A, Mansilla Eduardo, Goya Rodolfo G, Clarke Steven G Racemized and Isomerized Proteins in Aging Rat Teeth and Eye Lens Rejuvenation research, 2016; 19(4): 309-17.
Jain Kanishk, Warmack Rebeccah A, Debler Erik W, Hadjikyriacou Andrea, Stavropoulos Peter, Clarke Steven G Protein Arginine Methyltransferase Product Specificity is Mediated by Distinct Active-Site Architectures The Journal of biological chemistry, 2016; .
Lowenson Jonathan D, Shmanai Vadim V, Shklyaruck Denis, Clarke Steven G, Shchepinov Mikhail S Deuteration protects asparagine residues against racemization Amino acids, 2016; 19(4): .
Sherkhanov Saken, Korman Tyler P, Clarke Steven G, Bowie James U Production of FAME biodiesel in E. coli by direct methylation with an insect enzyme Scientific reports, 2016; 6(4): 24239.
Al-Hadid Qais, Roy Kevin, Chanfreau Guillaume, Clarke Steven G Methylation of yeast ribosomal protein Rpl3 promotes translational elongation fidelity RNA (New York, N.Y.), 2016; 22(4): 489-98.
Debler Erik W, Jain Kanishk, Warmack Rebeccah A, Feng You, Clarke Steven G, Blobel GÃ¼nter, Stavropoulos Pete A glutamate/aspartate switch controls product specificity in a protein arginine methyltransferase Proceedings of the National Academy of Sciences of the United States of America, 2016; 113(8): 2068-73.
Al-Hadid Qais, White Jonelle, Clarke Steven Ribosomal protein methyltransferases in the yeast Saccharomyces cerevisiae: Roles in ribosome biogenesis and translation Biochemical and biophysical research communications, 2016; 470(3): 552-7.
Fu Xudong, Chin Randall M, Vergnes Laurent, Hwang Heejun, Deng Gang, Xing Yanpeng, Pai Melody Y, Li Sichen, Ta Lisa, Fazlollahi Farbod, Chen Chuo, Prins Robert M, Teitell Michael A, Nathanson David A, Lai Albert, Faull Kym F, Jiang Meisheng, Clarke Steven G, Cloughesy Timothy F, Graeber Thomas G, Braas Daniel, Christofk Heather R, Jung Michael E, Reue Karen, Huang Jing 2-Hydroxyglutarate Inhibits ATP Synthase and mTOR Signaling Cell metabolism, 2015; 22(3): 508-15.
Yang Yanzhong, Hadjikyriacou Andrea, Xia Zheng, Gayatri Sitaram, Kim Daehoon, Zurita-Lopez Cecilia, Kelly Ryan, Guo Ailan, Li Wei, Clarke Steven G, Bedford Mark T PRMT9 is a type II methyltransferase that methylates the splicing factor SAP145 Nature communications, 2015; 6(27): 6428.
Hadjikyriacou Andrea, Yang Yanzhong, Espejo Alexsandra, Bedford Mark T, Clarke Steven G Unique Features of Human Protein Arginine Methyltransferase 9 (PRMT9) and Its Substrate RNA Splicing Factor SF3B2 The Journal of biological chemistry, 2015; 290(27): 16723-43.
Patananan Alexander N, Budenholzer Lauren M, Pedraza Maria E, Torres Eric R, Adler Lital N, Clarke Steven G The invertebrate Caenorhabditis elegans biosynthesizes ascorbate Archives of biochemistry and biophysics, 2015; 569(27): 32-44.
Patananan Alexander Nikolich, Budenholzer Lauren Michelle, Eskin Ascia, Torres Eric Rommel, Clarke Steven Gerard Ethanol-induced differential gene expression and acetyl-CoA metabolism in a longevity model of the nematode Caenorhabditis elegans Experimental gerontology, 2015; 61(27): 20-30.
Dzialo Maria C, Travaglini Kyle J, Shen Sean, Loo Joseph A, Clarke Steven G A new type of protein lysine methyltransferase trimethylates Lys-79 of elongation factor 1A Biochemical and biophysical research communications, 2014; 455(3-4): 382-9.
Feng You, Hadjikyriacou Andrea, Clarke Steven G Substrate specificity of human protein arginine methyltransferase 7 (PRMT7): the importance of acidic residues in the double E loop The Journal of biological chemistry, 2014; 289(47): 32604-16.
Dzialo Maria C, Travaglini Kyle J, Shen Sean, Roy Kevin, Chanfreau Guillaume F, Loo Joseph A, Clarke Steven G Translational roles of elongation factor 2 protein lysine methylation The Journal of biological chemistry, 2014; 289(44): 30511-24.
Al-Hadid Qais, Roy Kevin, Munroe William, Dzialo Maria C, Chanfreau Guillaume F, Clarke Steven G Histidine methylation of yeast ribosomal protein Rpl3p is required for proper 60S subunit assembly Molecular and cellular biology, 2014; 34(15): 2903-16.
Patananan Alexander N, Capri Joseph, Whitelegge Julian P, Clarke Steven G Non-repair pathways for minimizing protein isoaspartyl damage in the yeast Saccharomyces cerevisiae The Journal of biological chemistry, 2014; 289(24): 16936-53.
Lissina Elena, Weiss David, Young Brian, Rella Antonella, Cheung-Ong Kahlin, Del Poeta Maurizio, Clarke Steven G, Giaever Guri, Nislow Corey A novel small molecule methyltransferase is important for virulence in Candida albicans ACS chemical biology, 2013; 8(12): 2785-93.
Feng You, Maity Ranjan, Whitelegge Julian P, Hadjikyriacou Andrea, Li Ziwei, Zurita-Lopez Cecilia, Al-Hadid Qais, Clark Amander T, Bedford Mark T, Masson Jean-Yves, Clarke Steven G Mammalian protein arginine methyltransferase 7 (PRMT7) specifically targets RXR sites in lysine- and arginine-rich regions The Journal of biological chemistry, 2013; 288(52): 37010-25.
Yang Hongqian, Lowenson Jonathan D, Clarke Steven, Zubarev Roman A Brain proteomics supports the role of glutamate metabolism and suggests other metabolic alterations in protein l-isoaspartyl methyltransferase (PIMT)-knockout mice Journal of proteome research, 2013; 12(10): 4566-76.
Shen-Miller J, Lindner Petra, Xie Yongming, Villa Sarah, Wooding Kerry, Clarke Steven G, Loo Rachel R O, Loo Joseph A Thermal-stable proteins of fruit of long-living Sacred Lotus Nelumbo nucifera Gaertn var. China Antique Tropical plant biology, 2013; 6(2-3): .
Nayak Nihar R, Putnam Andrea A, Addepalli Balasubrahmanyam, Lowenson Jonathan D, Chen Tingsu, Jankowsky Eckhard, Perry Sharyn E, Dinkins Randy D, Limbach Patrick A, Clarke Steven G, Downie A Bruce An Arabidopsis ATP-dependent, DEAD-box RNA helicase loses activity upon IsoAsp formation but is restored by PROTEIN ISOASPARTYL METHYLTRANSFERASE The Plant cell, 2013; 25(7): 2573-86.
Mackay Kennen B, Tu Yiping, Young Stephen G, Clarke Steven G Circumventing Embryonic Lethality with Lcmt1 Deficiency: Generation of Hypomorphic Lcmt1 Mice with Reduced Protein Phosphatase 2A Methyltransferase Expression and Defects in Insulin Signaling PloS one, 2013; 8(6): e65967.
Patananan Alexander N, Palmer Jonathan M, Garvey Graeme S, Keller Nancy P, Clarke Steven G A novel automethylation reaction in the Aspergillus nidulans LaeA protein generates S-methylmethionine The Journal of biological chemistry, 2013; 288(20): 14032-45.
Clarke Steven G Protein methylation at the surface and buried deep: thinking outside the histone box Trends in biochemical sciences, 2013; 38(5): 243-52.
Dai Shujia, Ni Wenqin, Patananan Alexander N, Clarke Steven G, Karger Barry L, Zhou Zhaohui Sunny Integrated proteomic analysis of major isoaspartyl-containing proteins in the urine of wild type and protein L-isoaspartate O-methyltransferase-deficient mice Analytical chemistry, 2013; 85(4): 2423-30.
Dhar Surbhi, Vemulapalli Vidyasiri, Patananan Alexander N, Huang Grace L, Di Lorenzo Alessandra, Richard Stephane, Comb Michael J, Guo Ailan, Clarke Steven G, Bedford Mark T Loss of the major Type I arginine methyltransferase PRMT1 causes substrate scavenging by other PRMTs Scientific reports, 2013; 3(1): 1311.
Yang Mei-Ling, Gee Alaric J P, Gee Renelle J, Zurita-Lopez Cecilia I, Khare Shilpi, Clarke Steven G, Mamula Mark J Lupus autoimmunity altered by cellular methylation metabolism Autoimmunity, 2013; 46(1): 21-31.
Young Brian D, Weiss David I, Zurita-Lopez Cecilia I, Webb Kristofor J, Clarke Steven G, McBride Anne E Identification of methylated proteins in the yeast small ribosomal subunit: a role for SPOUT methyltransferases in protein arginine methylation Biochemistry, 2012; 51(25): 5091-104.
Castro Paola V, Khare Shilpi, Young Brian D, Clarke Steven G Caenorhabditis elegans battling starvation stress: low levels of ethanol prolong lifespan in L1 larvae PloS one, 2012; 7(1): e29984.
Zurita-Lopez Cecilia I, Sandberg Troy, Kelly Ryan, Clarke Steven G Human protein arginine methyltransferase 7 (PRMT7) is a type III enzyme forming Ï-NG-monomethylated arginine residues The Journal of biological chemistry, 2012; 287(11): 7859-70.
MacKay Kennen B, Lowenson Jonathan D, Clarke Steven G Wortmannin reduces insulin signaling and death in seizure-prone Pcmt1-/- mice PloS one, 2012; 7(10): e46719.
Adler Lital N, Gomez Tara A, Clarke Steven G, Linster Carole L A novel GDP-D-glucose phosphorylase involved in quality control of the nucleoside diphosphate sugar pool in Caenorhabditis elegans and mammals The Journal of biological chemistry, 2011; 286(24): 21511-23.
Webb Kristofor J, Al-Hadid Qais, Zurita-Lopez Cecilia I, Young Brian D, Lipson Rebecca S, Clarke Steven G The ribosomal l1 protuberance in yeast is methylated on a lysine residue catalyzed by a seven-beta-strand methyltransferase The Journal of biological chemistry, 2011; 286(21): 18405-13.
Khare Shilpi, Linster Carole L, Clarke Steven G The interplay between protein L-isoaspartyl methyltransferase activity and insulin-like signaling to extend lifespan in Caenorhabditis elegans PloS one, 2011; 6(6): e20850.
Petrossian Tanya C, Clarke Steven G Uncovering the human methyltransferasome Molecular & cellular proteomics : MCP, 2011; 10(1): M110.000976.
Webb Kristofor J, Zurita-Lopez Cecilia I, Al-Hadid Qais, Laganowsky Arthur, Young Brian D, Lipson Rebecca S, Souda Puneet, Faull Kym F, Whitelegge Julian P, Clarke Steven G A novel 3-methylhistidine modification of yeast ribosomal protein Rpl3 is dependent upon the YIL110W methyltransferase The Journal of biological chemistry, 2010; 285(48): 37598-606.
Lipson Rebecca S, Webb Kristofor J, Clarke Steven G Two novel methyltransferases acting upon eukaryotic elongation factor 1A in Saccharomyces cerevisiae Archives of biochemistry and biophysics, 2010; 500(2): 137-43.
Vinci Chris R, Clarke Steven G Homocysteine methyltransferases Mht1 and Sam4 prevent the accumulation of age-damaged (R,S)-AdoMet in the yeast Saccharomyces cerevisiae The Journal of biological chemistry, 2010; 285(27): 20526-31.
Webb Kristofor J, Lipson Rebecca S, Al-Hadid Qais, Whitelegge Julian P, Clarke Steven G Identification of protein N-terminal methyltransferases in yeast and humans Biochemistry, 2010; 49(25): 5225-35.
Fisk John C, Zurita-Lopez Cecilia, Sayegh Joyce, Tomasello Danielle L, Clarke Steven G, Read Laurie K TbPRMT6 is a type I protein arginine methyltransferase that contributes to cytokinesis in Trypanosoma brucei Eukaryotic cell, 2010; 9(6): 866-77.
Vinci Chris R, Clarke Steven G Yeast, plants, worms, and flies use a methyltransferase to metabolize age-damaged (R,S)-AdoMet, but what do mammals do? Rejuvenation research, 2010; 13(2-3): 362-4.
Petrossian Tanya, Clarke Steven Bioinformatic Identification of Novel Methyltransferases Epigenomics, 2009; 1(1): 163-175.
Khare Shilpi, Gomez Tara, Linster Carole L, Clarke Steven G Defective responses to oxidative stress in protein l-isoaspartyl repair-deficient Caenorhabditis elegans Mechanisms of ageing and development, 2009; 130(10): 670-80.
Lakowski Ted M, Zurita-Lopez Cecilia, Clarke Steven G, Frankel Adam Approaches to measuring the activities of protein arginine N-methyltransferases Analytical biochemistry, 2009; .
Petrossian, T. C., and Clarke, S. G. Bioinformatic Identification of Novel Methyltransferases Epigenomics, 2009; 1: 163-175.
Fisk John C, Sayegh Joyce, Zurita-Lopez Cecilia, Menon Sarita, Presnyak Vladimir, Clarke Steven G, Read Laurie K A type III protein arginine methyltransferase from the protozoan parasite Trypanosoma brucei The Journal of biological chemistry, 2009; 284(17): 11590-600.
Petrossian Laurie K, Clarke Laurie K Multiple motif scanning to identify methyltransferases from the yeast proteome Molecular & cellular proteomics : MCP, 2009; 284(17): .
Bedford Mark T, Clarke Steven G Protein arginine methylation in mammals: who, what, and why Molecular cell, 2009; 33(1): 1-13.
Gomez Tara A, Banfield Kelley L, Clarke Steven G The protein L-isoaspartyl-O-methyltransferase functions in the Caenorhabditis elegans stress response Mechanisms of ageing and development, 2008; 129(12): 752-8.
Linster Carole L, Clarke Steven G L-Ascorbate biosynthesis in higher plants: the role of VTC2 Trends in plant science, 2008; 13(11): 567-73.
Sayegh Joyce, Clarke Steven G Hsl7 is a substrate-specific type II protein arginine methyltransferase in yeast Biochem. Biophys. Res. Commun, 2008; 372(4): 811-5.
Banfield Kelley L, Gomez Tara A, Lee Wendy, Clarke Steven, Larsen Pamela L Protein-repair and hormone-signaling pathways specify dauer and adult longevity and dauer development in Caenorhabditis elegans The journals of gerontology. Series A, Biological sciences and medical sciences, 2008; 63(8): 798-808.
Dumlao Darren S, Hertz Nicholas, Clarke Steven Secreted 3-isopropylmalate methyl ester signals invasive growth during amino acid starvation in Saccharomyces cerevisiae Biochemistry, 2008; 47(2): 698-709.
Sayegh Joyce, Webb Kristofor, Cheng Donghang, Bedford Mark T, Clarke Steven G Regulation of protein arginine methyltransferase 8 (PRMT8) activity by its N-terminal domain J. Biol. Chem, 2007; 282(50): 36444-53.
Gomez Tara A, Clarke Steven G Autophagy and insulin/TOR signaling in Caenorhabditis elegans pcm-1 protein repair mutants Autophagy, 2007; 3(4): 357-9.
Linster Carole L, Gomez Tara A, Christensen Kathryn C, Adler Lital N, Young Brian D, Brenner Charles, Clarke Steven G Arabidopsis VTC2 encodes a GDP-L-galactose phosphorylase, the last unknown enzyme in the Smirnoff-Wheeler pathway to ascorbic acid in plants J. Biol. Chem, 2007; 282(26): 18879-85.
Vinci Chris R, Clarke Steven G Recognition of age-damaged (R,S)-adenosyl-L-methionine by two methyltransferases in the yeast Saccharomyces cerevisiae J. Biol. Chem, 2007; 282(12): 8604-12.
Gomez Tara A, Banfield Kelley L, Trogler Dorothy M, Clarke Steven G The L-isoaspartyl-O-methyltransferase in Caenorhabditis elegans larval longevity and autophagy Developmental biology, 2007; 303(2): 493-500.
Villa Sarah T, Xu Qilong, Downie A Bruce, Clarke Steven G Arabidopsis Protein Repair L-Isoaspartyl Methyltransferases: Predominant Activities at Lethal Temperatures Physiologia plantarum, 2006; 128(4): 581-592.
Clarke Steven G 16 Inhibition of mammalian protein methyltransferases by 5'-methylthioadenosine (MTA): A mechanism of action of dietary same? The Enzymes, 2006; 24(2): 467-93.
Farrar Christine E, Huang Christine S, Clarke Steven G, Houser Carolyn R Increased cell proliferation and granule cell number in the dentate gyrus of protein repair-deficient mice The Journal of comparative neurology, 2005; 493(4): 524-37.
Porras-Yakushi Tanya R, Whitelegge Julian P, Miranda Tina Branscombe, Clarke Steven A novel SET domain methyltransferase modifies ribosomal protein Rpl23ab in yeast The Journal of biological chemistry, 2005; 280(41): 34590-8.
Lee Jaeho, Sayegh Joyce, Daniel Jeremy, Clarke Steven, Bedford Mark T PRMT8, a new membrane-bound tissue-specific member of the protein arginine methyltransferase family The Journal of biological chemistry, 2005; 280(38): 32890-6.
Farrar Christine, Houser Carolyn R, Clarke Steven Activation of the PI3K/Akt signal transduction pathway and increased levels of insulin receptor in protein repair-deficient mice Aging cell, 2005; 4(1): 1-12.
Miranda Tina Branscombe, Miranda Mark, Frankel Adam, Clarke Steven PRMT7 is a member of the protein arginine methyltransferase family with a distinct substrate specificity The Journal of biological chemistry, 2004; 279(22): 22902-7.
Clarke Steven Aging as war between chemical and biochemical processes: protein methylation and the recognition of age-damaged proteins for repair Ageing research reviews, 2003; 2(3): 263-85.