Zhefeng Guo, Ph.D.
4155 Reed
UNITED STATES
Biography
Zhefeng Guo is a structural biologist and biochemist who joined the UCLA School of Medicine faculty in 2008. Dr. Guo earned his B.S. in Microbiology (1995) at Shandong University and M.S. in Biochemistry (1998) at Peking University in China. Then Dr. Guo came to the United States for graduate studies and received his Ph.D. in Biochemistry (2003) at UCLA, where he was focused on developing electron paramagnetic resonance (EPR) methods to study protein structure, dynamics, and folding in Wayne Hubbell’s laboratory. In 2004, Dr. Guo joined David Eisenberg’s research group for postdoc training, working on the structural characterization of amyloid fibrils using the approaches of protein engineering and X-ray crystallography. His laboratory is currently investigating the structure and fibrillization mechanism of amyloid fibrils related to a wide range of human disorders such as Alzheimer’s, Parkinson’s and prion diseases, with the goal of developing molecular diagnostics and therapeutics for amyloid diseases
Publications
- Jang C, Portugal Barron D, Duo L, Ma C, Seabaugh H, Guo Z. EPR Studies of Aβ42 Oligomers Indicate a Parallel In-Register β-Sheet Structure.. ACS chemical neuroscience, 2023.
- Portugal Barron D, Guo Z. The supersaturation perspective on the amyloid hypothesis.. Chemical science, 2023.
- Zhang A, Portugal Barron D, Chen EW, Guo Z. A protein aggregation platform that distinguishes oligomers from amyloid fibrils.. The Analyst, 2023.
- Chen EW, Guo Z. Preparation and Fractionation of Heterogeneous Aβ42 Oligomers with Different Aggregation Properties.. Methods in molecular biology (Clifton, N.J.), 2023.
- Xiao H, Duo L, Zhen J, Wang H, Guo Z. Static and dynamic disorder in Aβ40 fibrils.. Biochemical and biophysical research communications, 2022.
- Guo Z. Amyloid hypothesis through the lens of Aβ supersaturation.. Neural regeneration research, 2021.
- Gu L, Guo Z. Lipid membranes induce structural conversion from amyloid oligomers to fibrils.. Biochemical and biophysical research communications, 2021.
- Liu EN, Park G, Nohara J, Guo Z. Effect of spin labelling on the aggregation kinetics of yeast prion protein Ure2.. Royal Society open science, 2021.
- Yoon A, Zhen J, Guo Z. Segmental structural dynamics in Aβ42 globulomers.. Biochemical and biophysical research communications, 2021.
- Gu L, Guo Z. Alzheimer's Aβ42 and Aβ40 form mixed oligomers with direct molecular interactions.. Biochemical and biophysical research communications, 2020.
- Park G, Xue C, Wang H, Guo Z. Distinguishing the Effect on the Rate and Yield of Aβ42 Aggregation by Green Tea Polyphenol EGCG.. ACS omega, 2020.
- Wang H, Duo L, Hsu F, Xue C, Lee YK, Guo Z. Polymorphic Aβ42 fibrils adopt similar secondary structure but differ in cross-strand side chain stacking interactions within the same β-sheet.. Scientific reports, 2020.
- Wang J, Park G, Lee YK, Nguyen M, San Fung T, Lin TY, Hsu F, Guo Z. Spin Label Scanning Reveals Likely Locations of β-Strands in the Amyloid Fibrils of the Ure2 Prion Domain.. ACS omega, 2020.
- Xue C, Tran J, Wang H, Park G, Hsu F, Guo Z. Aβ42 fibril formation from predominantly oligomeric samples suggests a link between oligomer heterogeneity and fibril polymorphism.. Royal Society open science, 2019.
- Hsu F, Park G, Guo Z. Key Residues for the Formation of Aβ42 Amyloid Fibrils.. ACS omega, 2018.
- Wang H, Lee YK, Xue C, Guo Z. Site-specific structural order in Alzheimer's Aβ42 fibrils.. Royal Society open science, 2018.
- Xue C, Lee YK, Tran J, Chang D, Guo Z. A mix-and-click method to measure amyloid-β concentration with sub-micromolar sensitivity.. Royal Society open science, 2017.
- Xue C, Lin TY, Chang D, Guo Z. Thioflavin T as an amyloid dye: fibril quantification, optimal concentration and effect on aggregation.. Royal Society open science, 2017.
- Tran J, Chang D, Hsu F, Wang H, Guo Z. Cross-seeding between Aβ40 and Aβ42 in Alzheimer's disease.. FEBS letters, 2016.
- Gu L, Tran J, Jiang L, Guo Z. A new structural model of Alzheimer's Aβ42 fibrils based on electron paramagnetic resonance data and Rosetta modeling.. Journal of structural biology, 2016.
- Gu L, Liu C, Stroud JC, Ngo S, Jiang L, Guo Z. Antiparallel triple-strand architecture for prefibrillar Aβ42 oligomers.. The Journal of biological chemistry, 2014.
- Gu L, Liu C, Guo Z. Structural insights into Aβ42 oligomers using site-directed spin labeling.. The Journal of biological chemistry, 2013.
- Gu L, Guo Z. Alzheimer's Aβ42 and Aβ40 peptides form interlaced amyloid fibrils.. Journal of neurochemistry, 2013.
- Ngo S, Chiang V, Ho E, Le L, Guo Z. Prion domain of yeast Ure2 protein adopts a completely disordered structure: a solid-support EPR study.. PloS one, 2012.
- Agopian A, Guo Z. Structural origin of polymorphism of Alzheimer's amyloid β-fibrils.. The Biochemical journal, 2012.
- Ngo S, Chiang V, Guo Z. Quantitative analysis of spin exchange interactions to identify β strand and turn regions in Ure2 prion domain fibrils with site-directed spin labeling.. Journal of structural biology, 2012.
- Gu L, Ngo S, Guo Z. Solid-support electron paramagnetic resonance (EPR) studies of Aβ40 monomers reveal a structured state with three ordered segments.. The Journal of biological chemistry, 2012.
- Ngo S, Guo Z. Key residues for the oligomerization of Aβ42 protein in Alzheimer's disease.. Biochemical and biophysical research communications, 2011.
- Ngo S, Gu L, Guo Z. Hierarchical organization in the amyloid core of yeast prion protein Ure2.. The Journal of biological chemistry, 2011.
- López CJ, Fleissner MR, Guo Z, Kusnetzow AK, Hubbell WL. Osmolyte perturbation reveals conformational equilibria in spin-labeled proteins.. Protein science : a publication of the Protein Society, 2009.
- Guo Z, Eisenberg D. The structure of a fibril-forming sequence, NNQQNY, in the context of a globular fold.. Protein science : a publication of the Protein Society, 2008.
- Guo Z, Cascio D, Hideg K, Hubbell WL. Structural determinants of nitroxide motion in spin-labeled proteins: solvent-exposed sites in helix B of T4 lysozyme.. Protein science : a publication of the Protein Society, 2007.
- Guo Z, Cascio D, Hideg K, Kálái T, Hubbell WL. Structural determinants of nitroxide motion in spin-labeled proteins: tertiary contact and solvent-inaccessible sites in helix G of T4 lysozyme.. Protein science : a publication of the Protein Society, 2007.
- Guo Z, Eisenberg D. The mechanism of the amyloidogenic conversion of T7 endonuclease I.. The Journal of biological chemistry, 2007.
- Guo Z, Eisenberg D. Runaway domain swapping in amyloid-like fibrils of T7 endonuclease I.. Proceedings of the National Academy of Sciences of the United States of America, 2006.